12/03/2026
Artificial Insemination (AI) procedure in a cow, using the recto-va**nal method, which is the most common technique used in cattle reproduction.
Step-by-Step Procedure of Artificial Insemination (AI) in Cow
1️⃣ Restraining the Animal
• The cow is properly restrained in a chute or by rope to prevent movement.
• The tail is tied or held to the side.
• The v***a is cleaned with clean water or antiseptic to maintain hygiene.
2️⃣ Preparing the Semen Straw and AI Gun
• Frozen semen straw is taken from liquid nitrogen tank.
• It is thawed in warm water (35–37°C for about 30–40 seconds).
• The straw is loaded into the AI gun.
• The tip is cut and covered with a sterile sheath.
3️⃣ Re**al Manipulation (Recto-Vaginal Technique)
• The veterinarian inserts one gloved hand into the re**um.
• Through the re**al wall, the cervix is located and held.
• This helps guide the AI gun through the va**na to the cervix.
4️⃣ Passing the AI Gun Through the Cervix
• The AI gun is inserted through the v***a and va**na.
• Using the hand in the re**um, the cervix is manipulated over the AI gun.
• The gun is carefully passed through the cervical rings.
5️⃣ Semen Deposition in the Uterine Body
• When the gun tip reaches the uterine body, the semen is slowly deposited.
• The plunger is gently pushed to release semen.
• The AI gun is slowly removed afterward.
Veterinarian To Be
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